Eukaryotic Gene Expression Study Guide

Introduction:

• When and where certain genes are activated, and the amount of protein or RNA product generated is dependent on multiple factors.
• They are determined by a complex series of interactions between genes, RNA molecules, proteins (including transcription factors), and other components of the expression system.

The process of turning a gene on and off as per the cell’s requirements is known as gene regulation.

How is a Gene Regulated?

• By controlling the speed of transcribing. This is the most cost-effective way to regulate.
• By regulating RNA molecule processing, such as alternative splicing to create many protein products from a single gene. Messenger RNA (mRNA) molecule stability is regulated.
• By controlling the translation pace.

The Complexity of Eukaryotic Gene Regulation

Gene regulation is more complicated in eukaryotes than in prokaryotes. This is because their genomes are bigger and encode more genes. The genome of E. coli, for example, has around 5,000 genes, compared to approximately 25,000 genes in humans.

• Eukaryotes may create more than 25,000 proteins by splicing mRNAs in different ways and, in certain circumstances, starting transcription from different start sites in the same gene.
• Of course, without multigene operons, the activity of many more genes must be coordinated!
• Finally, eukaryotic gene regulation is confounded because all nuclear DNA in the form of chromatin is encased in protein.

Stages of Eukaryotic Gene Regulation

Many processes are involved in eukaryotic gene expression, and virtually all of them may be controlled. Different genes are regulated at different stages, and it's not unusual for a gene (especially one that's significant or powerful) to be regulated many times.

Chromatin accessibility:

The structure of chromatin (DNA and the proteins that organize it) can be controlled. A gene with more open or "relaxed" chromatin is more accessible for transcription.

Transcription:

For many genes, transcription is a critical regulatory point. Transcription factor proteins bind to certain DNA regions in or near genes, promoting or inhibiting their transcription into RNA.

RNA processing:

Splicing, capping, and adding a poly-A tail to an RNA molecule, as well as egress from the nucleus, may all be controlled. Alternative splicing can produce different mRNAs from the same pre-mRNA.

Check out our transcription simulation to experience RNA processing in VR!

RNA stability:

The number of proteins generated from an mRNA molecule depends on how long it spends in the cytosol. MiRNAs, which are small regulatory RNAs, can bind to target mRNAs and cause them to be split up.

Translation:

Regulators can boost or stifle the translation of an mRNA. For example, MicroRNAs (MiRNAs) can occasionally prevent their target mRNAs from being translated (rather than causing them to be chopped up).

Check out our Translation simulation to learn more about this process!

Protein modifications:

Proteins can be broken apart or labeled with chemical groups, among other things. These changes may be controlled, and they can impact the protein's activity or behavior.

Eukaryotic Gene Regulation by Hormones

• Complete pathways have developed in eukaryotic organisms that respond to external or planned developmental signals and result in the right cellular response.
• Chemicals that control genes in eukaryotes are seldom transmitted to neighboring cells as signals.
• Chemicals secreted by some cells in eukaryotes signal other cells to respond, coordinating the entire organism's activity.
• Hormones are well-understood signal molecules generated by endocrine glands; hormones impact target cells throughout the body.

Eukaryotic Gene Regulation by Steroid Hormones

• To execute their effects, steroid hormones must get through cell membranes.
• Testosterone, estrogens, progesterone, glucocorticoids, and mineralocorticoids are all examples of steroid hormones.
• Such hormones bind to a steroid hormone receptor protein in the target cell to create a steroid hormone-receptor complex.
• The receptor can be found in the cytoplasm or the nucleus. Still, to increase or mute transcription, the hormone-receptor complex must attach to DNA regulatory regions of a gene.
• As a result, a steroid hormone must pass through the plasma membrane and perhaps the nuclear envelope.

Eukaryotic Gene Regulation by Protein Hormones:

• Protein hormones, by definition, are big, soluble, and have highly charged surfaces.
• Other hormones, such as adrenalin, may be small, but they are charged.
• The phospholipid barrier of the plasma membrane prevents large or highly charged signal molecules from passing through.
• They must bind to receptors on the surface of cells to have any impact. Membrane glycoproteins are commonly used as receptors.
• Signal transduction is when information (signals) contained by protein hormones is transferred into the cell indirectly.

There are two well-known signal transduction mechanisms, both of which entail activating protein phosphorylation processes in the cytoplasm. A transcription factor that binds to regulatory DNA is activated due to the phosphorylation cascade, which turns a gene on or off.

Conclusion:

• The biological mechanisms governing the pace and method of gene expression in eukaryotes are called gene regulation.
• Gene expression is controlled at the epigenetic, transcriptional, post-transcriptional, translational, and post-translational levels in eukaryotic cells.
• Gene regulation is more complicated in eukaryotes than in prokaryotes

FAQs:

1. What are the steps in eukaryotic gene regulation?

• Epigenetic controls at the DNA level include DNA methylation, histone changes, chromatin remodeling, and RNA-based regulation. Then there are a variety of transcription start sites to choose from.
• Multiple splice variants, miRNA, and siRNA exist at the RNA level.
• Post-translational changes at the protein level

2. What is an example of eukaryotic gene expression?

The TATA box, for example, is named for its core sequence of TATAAA. Most eukaryotic genes include this regulatory region as part of their promoter.

3. What is the purpose of gene regulation in eukaryotes?

The process of turning genes on and off is known as gene regulation. Cells begin to take on distinct tasks throughout early development. Gene regulation ensures the right genes are expressed at the right times. Gene control can also aid an organism's ability to respond to its surroundings.

4. What are the five levels of genetic regulatory control in eukaryotes?

Gene expression is controlled at the epigenetic, transcriptional, post-transcriptional, translational, and post-translational levels in eukaryotic cells.

5. What is the eukaryotic gene?

Eukaryotic genes are DNA sections that serve as templates for RNA polymerases to produce RNA.

6. What is the most important level of gene regulation in eukaryotes?

In both prokaryotic and eukaryotic cells, sequence-specific transcription factors are the most significant and varied means of gene control.

7. What is the first level of gene regulation in eukaryotes?

The epigenetic level is the initial level of gene expression regulation in eukaryotic cells. Access to the chromosomal area is controlled by epigenetic processes, which allow genes to be switched on or off.

8. Why are most eukaryotic genes off?

Gene expression is defaulted to "off" in eukaryotes rather than "on" as in prokaryotes. The key is chromatin, which is a cellular nucleus-wide combination of DNA and histone proteins. Histones are among the most evolutionarily conserved proteins; they are essential for eukaryotes' survival and tolerate little alteration. When a gene is tightly bonded with histone, it is said to be "off."

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